|
KMID : 0371320070730030235
|
|
Journal of the Korean Surgical Society
2007 Volume.73 No. 3 p.235 ~ p.241
|
|
|
Optimal Number of Hepatocytes per Microcarrier in Spheroid Culture using Cytodex 3 Microcarrier
|
|
Shin Woo-Young
Lee Kuhn-Uk
Lee Hae-Won
Cho Eung-Ho
Yi Nam-Joon
Suh Kyung-Suk
|
|
|
Abstract
|
|
|
Purpose:The mass cultivation of functional hepatocytes is a key factor of a bioartificial liver. Combining spheroid and microcarrier cultures has been applied for enhancing the cell viability and metabolic activities. Hence, the optimal number of hepatocytes per microcarrier was investigated.
Methods:Firstly, spheroid cultures were carried out with 1 g Cytodex 3 microcarrier plus 2×109, 4×108 and 8×107 viable hepatocytes per flask. The numbers of hepatocytes per microcarrier were approximately 666.7, 133.3 and 26.7, respectively. The control group consisted of a spheroid culture of 4×108 hepatocytes without any microcarrier. According to the primary experimental results, spheroid cultures with 1×108 of hepatocytes plus 1 g, 2 g and 3 g of the Cytodex 3 microcarrier were performed. The numbers of hepatocytes per microcarrier were approximately 33.3, 16.7 and 11.1, respectively. The control group consisted of a spheroid culture of 1×108 hepatocytes. The cell viabilities were assayed using a Cell Counting Kit-8; with the albumin production assayed using ELISA.
Results:According to the primary experiment, the group consisting of 26.7 hepatocytes per microcarrier showed the highest viability (P＜0.01). However, there was no statistical difference in the albumin production between the groups (P=0.744). The second Experiment showed the groups consisting of 11.1 and 16.7 hepatocytes per microcarrier had higher viabilities than the other hepatocyte and control groups (P＜0.01). The albumin production was similar for each group (P=0.187).
Conclusion: With respect to their application to a bioartificial liver, about 130 hepatocytes per microcarrier was appeared to be good for the mass cultivation of a hepatocytes spheroid culture using the Cytodex 3 microcarrier.
|
|
|
KEYWORD
|
|
|
Hepatocyte, Spheroid culture, Microcarrier
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|